Natural Science 0388 - Adv Methods in Molecular Bio
Fall
2013
1
4.00
John Castorino
02:30PM-05:30PM M;02:30PM-05:30PM M
Hampshire College
312229
Cole Science Center 2-OPEN;Cole Science Center 2-MOLC
jcNS@hampshire.edu
This course is intended for Div III and advanced Div II students who are committed to proposing, developing, or carrying-through research projects involving techniques commonly used in a molecular biology lab. Students will learn intermediate to advanced approaches, protocols, and strategies for molecular biology. Depending on student projects, methods may include advanced PCR (inverse, nested, multiplexed, primer-based mutatagenesis), qPCR, genomic and cDNA library construction, RNA and protein expression, hybridization methods, cloning and transformation strategies, imaging, cell-tissue culture, bioinformatics, and assessment of genomic variation. Readings will draw from primary literature, and students will present project reports in written and oral formats. Students may explore projects in development of, or in conjunction with, Div III work or independent studies. Selected students will develop projects in preparation for TAing a spring lab course in basic molecular biology methods. Prerequisites: Methods in Molecular Biology or equivalent lab experience is required.
Independent Work Quantitative Skills Writing and Research
Multiple required components--lab and/or discussion section. To register, submit requests for all components simultaneously.
This course has unspecified prerequisite(s) - please see the instructor.