Biology 314 - T-ADV MICROSCOPY TECHNIQ: LSCM

Topics course.Instrument specific topics course designed for research students (special studies, honors, SURF, etc.) requiring access to microscope equipment in the Center for Microscopy and Imaging (CMI). Each semester, three six-week courses are offered. All students meet the first two weeks to discuss their projects and the last week to present their work. During the remaining three, students learn how to operate a microscope independently (see topics). Evaluation will be through engagement in assigned activities. 400 level work cannot overlap with this course work. Prerequisite: Permission of the Instructor. Enrollment limited to 6 per topic. Graded S/U only.: The LSCM is used to study fluorescently labelled live or fixed cells, tissues, and small organisms. By blocking the out-of-focus light, the image quality is improved compared to widefield fluorescence microscopy. Optical sections of materials can be collected and used to reconstruct the three-dimensional structure. Training includes a lecture, demonstrations, discussions, and hands-on microscope instruction. Participants will meet for a total of 6 weeks during the semester.

Instructor Permission.