Biology 314 - T-ADV MICROSCOPY TECHNQ: TIRFM
Fall
2018
03
1.00
Judith Wopereis
F 01:10-04:00
Smith College
10698-F18
SAB-RD 314
jwoperei@smith.edu
Topics course.Instrument specific topics course designed for research students (special studies, honors, SURF, etc.) requiring access to microscope equipment in the Center for Microscopy and Imaging (CMI). Each semester, three six-week courses are offered. All students meet the first two weeks to discuss their projects and the last week to present their work. During the remaining three, students learn how to operate a microscope independently (see topics). Evaluation will be through engagement in assigned activities. 400 level work cannot overlap with this course work. Prerequisite: Permission of the Instructor. Enrollment limited to 6 per topic. Graded S/U only.: The TIRFM is used to study fluorescently labelled molecules in a very thin region of the sample adjacent
to the coverglass. With a depth of up to ~100nm, this technique is well-suited to examine, for example,
individual molecules, cell membranes, and other cell surface components and processes. Training
includes a lecture, demonstrations, discussions, and hands-on microscope instruction. Participants will
meet for a total of 6 weeks during the semester.
to the coverglass. With a depth of up to ~100nm, this technique is well-suited to examine, for example,
individual molecules, cell membranes, and other cell surface components and processes. Training
includes a lecture, demonstrations, discussions, and hands-on microscope instruction. Participants will
meet for a total of 6 weeks during the semester.
Instructor Permission.