Biology 314 - T-ADV MICROSCOPY TECHNIQ:TIRFM

Topics course.Instrument specific topics course designed for research students (special studies, honors, SURF, etc.) requiring access to microscope equipment in the Center for Microscopy and Imaging (CMI). Each semester, three six-week courses are offered. All students meet the first two weeks to discuss their projects and the last week to present their work. During the remaining three, students learn how to operate a microscope independently (see topics). Participants are expected to meet outside of class for weekly hands-on training sessions. Only one topic per semester can be selected. Evaluation will be through engagement in assigned activities. 400 level work cannot overlap with this course work. Permission of the Instructor. Enrollment limited to 6 per topic. Graded S/U only: The TIRFM is used to study fluorescently labelled molecules in a very thin region of the sample adjacent to the coverglass. With a depth of up to ~100nm, this technique is well-suited to examine, for example, individual molecules, cell membranes, and other cell surface components and processes. Training includes a lecture, demonstrations, discussions, and hands-on microscope instruction. Participants will meet for a total of 6 weeks during the semester. (E)

Instructor Permission.