Biology 314 - T-ADV MICROSCOPY TECHNIQ: TEM

Topics course.Instrument specific topics course designed for research students (special studies, honors, SURF, etc.) requiring access to microscope equipment in the Center for Microscopy and Imaging (CMI). Each semester, three six-week courses are offered. All students meet the first two weeks to discuss their projects and the last week to present their work. During the remaining three, students learn how to operate a microscope independently (see topics). Participants are expected to meet outside of class for weekly hands-on training sessions. Only one topic per semester can be selected. Evaluation will be through engagement in assigned activities. 400 level work cannot overlap with this course work. Permission of the Instructor. Enrollment limited to 6 per topic. Graded S/U only: The TEM is used to study morphological features of large molecules, cells, tissues, small organisms, and particles at a resolution of 0.3 nm. Ultrathin sections (~100nm) need to be cut to study internal features of cells and tissue. Negative staining techniques can be applied to observe large molecules, small organisms, and particles. Training includes a lecture, demonstrations, discussions, and hands-on microscope instruction. Mechanical and optical components will be reviewed and operational parameters improving image quality covered.

Instructor Permission.