Biology 314 - Topics in Advanced Microscopy Techniques for Research: Total Internal Reflection Fluorescence Microscopy

Instrument specific topics course designed and highly recommended for research students (special studies, honors, SURF, etc.) requiring future access to microscope equipment in the Center for Microscopy and Imaging (CMI). Students will discuss the need of the Microscope system of their choice for their research project by preparing and presenting short presentations and a poster (or alternative format). Emphasized are group and/or individual demonstration sessions required to learn about the principles and operating parameters of a microscope system (see topics). Digital Imaging and Image Analysis Techniques will be discussed. Evaluation will be through engagement in assigned activities. 400 level work cannot overlap with this course work. Instructor permission only. Enrollment limited to 6. S/U only: The TIRFM is used to study fluorescently labelled molecules in a very thin region of the sample adjacent to the coverglass. With a depth of up to ~100nm, this technique is well-suited to examine, for example, individual molecules, cell membranes, and other cell surface components and processes. Mechanical and optical components will be reviewed and operational parameters improving image quality covered. Instruction includes lectures, demonstrations and discussions and substitutes part of CMI training towards independent microscope use.

Instructor Permission.