Biology 314ls - Topics in Advanced Microscopy Techniques-Laser Scanning Confocal Microscopy

Adv Microscopy:T-LSCM

Fall
2021
01
1.00
Judith Lidwina-Maria Wopereis

F 1:10 PM - 4:00 PM

Smith College
BIO-314ls-01-202201
Sabin-Reed 411
jwoperei@smith.edu
The LSCM is used to study fluorescently labelled live or fixed cells, tissues, and small organisms. By blocking the out-of-focus light, the image quality is improved compared to widefield fluorescence microscopy. Optical sections of materials can be collected and used to reconstruct the three-dimensional structure. Mechanical and optical components will be reviewed and operational parameters improving image quality covered. Instruction includes lectures, demonstrations, discussions and hands-on activities and will count towards some of the CMI training to become an independent microscope user.  Only one topic per semester can be selected. 400 level work cannot overlap with this course work. Enrollment limited to 6 per topic. Graded S/U only.
Permission is required for interchange registration during the add/drop period only.
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