Biology 314ls - Topics in Advanced Microscopy Techniques-Laser Scanning Confocal Microscopy

The LSCM is used to study fluorescently labelled live or fixed cells, tissues, and small organisms. By blocking the out-of-focus light, the image quality is improved compared to widefield fluorescence microscopy. Optical sections of materials can be collected and used to reconstruct the three-dimensional structure. Mechanical and optical components will be reviewed and operational parameters improving image quality covered. Instruction includes lectures, demonstrations and discussions and substitutes part of CMI training towards independent microscope use. Enrollment limited to 12.