Biology 314te - Topics in Advanced Microscopy Techniques-Transmission Electron Microscopy

The TEM is used to study morphological features of large molecules, cells, tissues, small organisms, and particles at a resolution of 0.3 nm. Ultrathin sections (~100nm) need to be cut to study internal features of cells and tissue. Negative staining techniques can be applied to observe large molecules, small organisms, and particles. Mechanical and optical components will be reviewed and operational parameters improving image quality covered. Instruction includes lectures, demonstrations and discussions and substitutes part of CMI training towards independent microscope use. Enrollment limited to 6.