Microbiology 542 - Immunology Lab

Laboratory procedures in cellular immunology and immunochemistry. Lab procedures explore protein chemistry of antibodies, including antibody isolation using salt precipitation, ion exchange and molecular sieving column chromatography, spectrophotometry, SDS polyacrylamide gel electrophoresis (PAGE), western blotting, immunoprecipitation, Immunodiffusion (Ouchterlony technique) and enzyme-linked immunoassay (ELISA) and Immunofluorescence staining. Anatomy of the lymphatic system, mouse dissection and isolation of lymphocytes from spleen and thymus; cellular immunology, including histology of leukocytes (mouse and human), normal and diseased lymphoid tissue, and two-color flow cytometric analysis of lymphocyte subpopulations. Prerequisite or corequisite: course in immunology e.g., MICROBIO 320 or MICROBIO 540. Mr. Webley