Animal Science 385 - Biotechnology Laboratory

Spring
2018
01
4.00
Dominique Alfandari
M W 8:00AM 12:00PM
UMass Amherst
54609
Students will receive practical experience in basic and advanced cell and molecular biology techniques used in biotechnology and research laboratories. The course will be divided in two modules. The first module, mammalian cell culture, will utilize two breast cancer cell lines to examine the role of DNA methylation in gene expression and ultimately disease. The second module will train students in the use of fluorescent microscopy to visualize organelles in living cells, in methods of protein purification and analysis, and in the use of luciferase reporter assays to measure transcriptional activity. In addition to providing students with modern biological laboratory skills, this course is designed to encourage students to think critically in the analysis of experimental results.
ANIMLSCI 200 & 311 + BIOCH/BIO Throughout the semester students will be encouraged to discuss results and trouble-shoot problems as they arise. Students also will practice careful note-taking, organizing laboratory reports, and presentation skills.

Additional fees are associated with this section
Permission is required for interchange registration during the add/drop period only.