Chemical Engineering 690B - Biotech Lab Modules

Laboratory Modules are hands-on training in interdisciplinary and translational experimental, analytical, and computational techniques. Modules emphasize developing and/or adapting tools to better serve the biotechnology industry. Modules are centered around a particular technique, but they fundamentally stress how these techniques can be applied to a variety of different problems, perhaps changing the way students think about how they approach certain problems, and include what opportunities and constraints techniques have, with a focus on the rigor and reproducibility associated with said technique.

Open to graduate students in STEM fields. Priority will be given to students in the BTP and CBI training programs & additional students allowed as space is available.

Assessment of Macrophage Repolarization using Flow Cytometry. (Chem Eng 690B - Sec 02) Instructor: Ashish Kulkarni Oct 27-28
Macrophages are the essential component of our innate immune system and play a central role in various physiological processes, including defense against pathogens, clearance of cellular debris, tissue remodeling and repair. To fulfill these functionally distinct roles, macrophages are capable of polarizing toward a multitude of phenotypes based on the environment they encounter, especially the M1 pro-inflammatory phenotype and immunosuppressive M2 phenotype. In this lab module, the students will assess the effect of different agents on macrophage repolarization from an M2 phenotype to an M1 phenotype. In addition, they will evaluate the extent of macrophage repolarization after different treatments using the flow cytometry technique. This module will cover the basic concepts in macrophage biology and their roles in different diseases. Space in the course is limited (12 student cap).