Reports and discussion of pertinent literature and research. Required of all microbiology graduate majors each semester in residence.

Individualized Study. Research under direction of a faculty member.

The Applied Molecular Biotechnology Laboratory (AMBL) is a requisite course for the MS concentration in Applied Molecular Biology. AMBL combines state-of-the-art laboratory training with independent research experience to train students in the latest techniques and concepts of molecular biology and biochemistry. Recombinant DNA technology is the primary focus of the first semester. Topics covered include nucleic acid isolation, genomics, quantitative PCR and RT-PCR, southern and northern blot analysis, and computational biological analyses.

The main aspects of bacterial growth, including energy metabolism, biosynthesis of macromolecular precursor materials and their assembly into macromolecules, and the integration of these processes by various regulatory mechanisms. Emphasis on the isolation of mutant bacteria blocked in key cellular functions and on global control systems governing the adaptation of bacteria to different environmental conditions. Prerequisite: general background in microbiology and biochemistry.

Individualized study. Research under direction of faculty member.

Methodology and principles of modern molecular genetics. Microbial genetics combining classical techniques with bacteriophages and bacteria with modern PCR and recombinant DNA experiments. Introduction to genomic and structural analysis using computer methods. Gene expression in insect tissue culture cells using the baculovirus gene expression system. Designed to help students learn techniques and analyze results.

This course covers a detailed analysis of the molecular mechanisms that control the maintenance, expression, and evolution of prokaryotic and eukaryotic genomes. The topics covered in lectures and readings of relevant literature include DNA replication, transcription, gene regulation, genetic recombination, and translation. Class format will include lectures, journal clubs, presentations and group discussions.

Laboratory procedures in cellular immunology and immunochemistry. Lab procedures explore protein chemistry of antibodies, including antibody isolation using salt precipitation, ion exchange and molecular sieving column chromatography, spectrophotometry, SDS polyacrylamide gel electrophoresis (PAGE), western blotting, immunoprecipitation, Immunodiffusion (Ouchterlony technique) and enzyme-linked immunoassay (ELISA) and Immunofluorescence staining.

Not available at this time

Not available at this time